Journal: Engineering in Life Sciences

Article Title: Evaluating feeding strategies for microbial oil production from glycerol by Rhodotorula glutinis

doi: 10.1002/elsc.201600073

Figure Lengend Snippet: Comparison of cultivation modes employed for single oil production from glycerol by oleaginous yeasts

Article Snippet: In contrast to exponential feeding, pulsed and constant feedings were more efficient for growth and lipid production. table ft1 table-wrap mode="anchored" t5 Table 3 caption a7 Yeast strain Carbon source Cultivation mode Cultivation scale DCW (g/L) Oil content (% w/w) Oil titer (g/L) Y L/Gly (g/g) Q oil (g/L/h) Reference C. curvatus ATCC 20509 Pure glycerol Fed‐batch: two‐stage Bioreactor 118 25 29.5 0.11 0.59 15 R. glutinis CICC 31596 Pure glycerol Fed‐batch: two‐stage Bioreactor 16.8 34.6 5.07 0.03 0.03 22 R. toruloides Y4 Pure glycerol Batch Bioreactor 35.3 46.0 16.2 0.26 0.14 14 Y. lipolytica MUCL 28849 Pure lycerol a Fed‐batch: two stage Bioreactor 40.9 38.4 15.73 0.17 0.33 23 R. toruloides Y4 Pure glycerol Fed‐batch: two‐stage Shake‐flask 21.1 40.3 8.5 0.22 0.07 42 R. glutinis TISTR 5159 Crude glycerol Fed‐batch Bioreactor 10.5 60.7 6.1 0.06 0.12 45 R. toruloides Y‐27012 Crude glycerol Batch Bioreactor 30.1 34 10.2 0.07 0.28 46 Y. lipolytica LGAM S(7)1 Crude glycerol Continuous Bioreactor 8.1 43 3.5 0.09 0.11 17 C. freyschussii ATCC 18737 Pure glycerol Fed‐batch (glycerol pulses) Bioreactor 30.5 30 9.1 0.08 0.03 24 C. freyschussii ATCC 18737 Pure glycerol Fed‐batch (continuous feeding) c Bioreactor 82 34 28 0.07 1.8 24 Y. lipolytica JMY 4086 Crude glycerol b Fed‐batch: two stage (continuous feeding) c Bioreactor 49.1 46 22.6 0.08 0.31 31 R. glutinis BCRC 21418 Crude glycerol Fed‐batch (continuous glycerol supply) Bioreactor 44.8 62.1 27.82 ‐ 0.45 28 R. glutinis BCRC 21418 Crude glycerol Fed‐batch (exponential glycerol supply) Bioreactor 39.2 43.3 16.97 ‐ 0.23 28 R. glutinis CICC 31596 Pure glycerol Fed‐batch: two stage (pulsed) Bioreactor 23 40.8 9.38 0.059 0.06 This study R. glutinis CICC 31596 Pure glycerol Fed‐batch: two stage (continuous feeding) d Bioreactor 24.23 39.4 9.55 0.06 0.06 This study R. glutinis CICC 31596 Pure glycerol Fed‐batch: two stage (continuous feeding) d Bioreactor 28 41.5 11.38 0.061 0.07 This study R. glutinis CICC 31596 Pure glycerol Fed‐batch: two stage (continuous feeding) d Bioreactor 30.63 53 16.28 0.087 0.10 This study Open in a separate window a Pure glycerol was used only during the growth stage. b Crude glycerol was fed only during the lipid stage. c Glycerol and nutrients were fed throughout the fermentation. d Glycerol and yeast extract in the first stage, glycerol only feeding in the second stage.

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